How to Apply Gel Stain and Gel Topcoat to Raw Wood. General Finishes.  Application Tools, Techniques & Tips | FB Live with Bill Strey. Stain-free gels incorporate the trihalo compound in their gel formulation and are run with standard protocols and reagents like any other gel used in SDS-PAGE. Unlike with Coomassie or other dyes, there is no destaining step, and the stain-free technology is environmentally safe and does not generate toxic, hazardous organic waste.  Stain-free technology is compatible with most downstream applications, which helps not only when resources are limited, but also in the appropriate normalization of data for quantitation (see below). Verification of Protein Transfer.  However, the technique does require an imager that can visualize the activated gel or membrane. How Is Stain-Free Technology Likely to Affect the Way We Do Protein Research Today?. Stain Compatibility for Bio-Rad Gel Documentation Systems. Choose the best imaging system for your lab based on your most important applications. Nucleic Acid Detection. Gel Documentation System.  Stain-free western blotting allows you to quickly check electrophoresis and blot transfer quality and obtain truly quantitative western blotting results, updating traditional blotting techniques with innovative tools. Faster Results. Better Data.  Find the right Bio-Rad protein gel for your application. Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries. Image Lab Software. Unless you are finishing a quality hardwood, such as oak, mahogany or walnut (not cherry, it blotches), you will be safest using a gel stain. Gel stains are thick and very effective at reducing blotching (uneven coloring due to inconsistent densities in the wood). No matter which stain you use, the method of application is the same. Correct Application. Wood stain can be applied with a paintbrush or foam pad, or it can be rubbed in with a clean cloth. Your choice of methods depends partly on your personal preference, but each type of stain is best suited to certain methods. If you're going to use a gel stain, apply it with a rag and wipe it down once the desired color is. Apply the gel stain liberally with either a cotton cloth, natural bristle brush or a poly foam brush. (Be sure to test a small area of your project to make sure it is the finish you want and that the application method you've chosen is working well for you.) Wipe off the gel stain going with .
Despite the awful picture no natural light , you can hopefully see that this transforma…. I like to use High Performance Top Coat water-based over this stain. The time needed to view the stain on the blot also varies with the dye used. And yes, go do it!!! I want to go for a gray oak stain.

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