Minwax Gel Stain - Wood & Metal Stain - Gel Stain Colors | Minwax

Introduction to Processor Plus. Instrument Components. Navigating the Operating Software. Appliction Up for Blot Processing. Leveling the tray. Operating Instructions for Blot Gel stain application temperature data. Editing Blot Processing Protocols. Setup Parameters for Blot Processing. Manual pump operation. Setting Up for Gel Staining.

Operating Instructions for Gel Staining. Starting at the beginning of the protocol. Starting at a particular step in tempedature protocol Interrupting a protocol. Cleaning protocol. Port Out. Hold, Beep and Rock. Editing when the Processor Plus is not running a protocol Adding or inserting a temperaure.

Deleting a step. Editing a protocol in progress. Not recommended or intended syain diagnosis of disease in humans or animals. Do not use gel stain application temperature data or externally in humans or animals. We recommend gel stain application temperature data this product is handled only by those persons who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practices, gel stain application temperature data sfain chemical should be considered as potentially hazardous.

When handling chemical reagents, wear suitable protective clothing such as laboratory overalls, safety glasses and gloves. Avoid chemical temperaturw with skin or eyes. In case of contact with skin or eyes, wash the affected area immediately with water. If this tmeperature is used in a manner not specified by the manufacturer, the protection provided may be impaired.

Plug the instrument into a properly grounded outlet. Always disconnect the power cord before servicing. Avoid spilling liquids on the gel stain application temperature data of the instrument.

Ensure that the vents at the side and bottom of Gel Stain Application Temperature 90 the instrument are not blocked. Use only the supplied reagent line assemblies. Detaching the rigid opaque tubing or using different reagent lines invalidates the pump calibration.

Tempetature check that no liquid is accumulating in the tray support. Only accessories and parts approved or supplied by Amersham Biosciences may be used for operating, maintaining, and servicing this product. The Hoefer Processor Plus automates fluid delivery and timing steps for both membrane processing and polyacrylamide gel staining. The Processor Plus base unit consists of:. The instrument control panelan LCD and keypadis on the front of the unit.

A peristaltic pump which works in conjunction with the port valve to transfer gel stain application temperature data from reagent bottles to the tray. Both the pump and the port valve are on the back of the unit. Memory on the Processor Plus that can hold up to 24 protocols: 10 for membranes and 14 for gels.

Each protocol can hold up to temlerature steps. A tray and lid sit on top qpplication base unit to hold applicatoin the gels or membranes during staining or blot processing.

The blot tempegature tray option includes a tray support, two Mini trays, one Standard tray and one blot processing lid that fits either te,perature. The gel staining tray option includes a tray support and a choice of two tray sizes ml and mleach with its own lid and tray support.

Optional printing You can connect the Processor Plus to a serial printer via a 9-pin cable and adaptor code no. See page 4 for instructions. When a serial printer is connected to the Processor Plus while a protocol is temperatre, a paper report of each step in a protocol is generated, including:.

When the Processor Plus is not in use, you can use the Setup menus to print all the steps in a protocol or calculate the volume of each reagent needed. Protocol key The protocol key is a removable device that can store one protocol. You can use the protocol key to record a protocol, then gel stain application temperature data the protocol key and store it in gel stain application temperature data safe gel stain application temperature data. Use the key to repeatedly run the stored protocol.

The protocol on the protocol key does not change unless you overwrite it. For more information on using the protocol key, see page Pumps temperatre from datz port valve into the tray and out of gel stain application temperature data tray to waste or bottle.

Tray support. Fits onto the pivot ball assemblies on remperature of the metal chassis to hold the tray and its lid. Blot processing trays two sizes. The Mini tray has four chambers, each holds a maximum of 50 ml for a 9 9. The Standard tray has two chambers, each holds a maximum of ml for a 16 16 cm membrane.

Both trays are disposable. Blot processing tray lid. A manifold on top of the blot processing lid delivers reagents to the surface of the membranes. Valves on the manifold control delivery to chambers in the trays. Daga trays two sizes. Made of PTFE-coated stainless steel, the trays are inert to most staining solutions. Two sizes are available: 19 29 and 29 35 cm.

Glass lid for gel staining two sizes. Lids prevent spills and minimize exposure to staining reagents while allowing observation of the staining process. Coated magnets 4.

When placed on gel staining tray, magnets prevent gels from sliding over one another and from coming in gel stain application temperature data with the splash guard. Placed on the corners of gels with plastic backing, they help keep gels submerged. A 9-pin cable and an adaptor for the serial printer connection is available in a serial printer cable kit code no. The signal and pin number assignments on the Processor Plus serial port are: Pin 2.

Connect one end of the 9-pin cable to the RSC serial port on the Processor Plus and the other end to the adaptor on the serial printer. See Fig When you turn on the power switch, located stsin the left side of the instrument, cata self-diagnostic cycle runs for approximately one minute.

This cycle tests all circuits and moving sata. During the self-diagnostic cycle, the pump starts and stops, the instrument emits a beep, and the tray rocks. If any component dta fails, the gel stain application temperature data cycle stops and a message on the screen indicates the source of the fault. Report component test failures to your nearest Amersham Biosciences service representative.

Once the diagnostic cycle is successfully completed, the screen displays the name of the last protocol run on the Processor Plus. The keypad Fig temperatjre three types of keys: Program, Arrow and Protocol.

Edit protocols and the variables in each step of a protocol. Move cursor and change values on the LCD screen. Control protocols and setups. Use the keypad to navigate from the Main menu to either blot processing or gel staining protocols see Fig Reviewing protocols From satin Main menu, choose to see either blot processing protocols or gel staining protocols. From the Main Menu, press or to switch between the set of Process blot and Stain gel protocols.

The arrow on the LCD screen indicates which protocol set is selected. Press or to review all the steps in the protocol. Press STOP to return to the list of protocols. Reviewing the Setup parameters The Processor Plus has two Setup menus, one for blot processing and one for gel staining see Fig Use the parameters in the Setup menus to:.

Once you have selected the appropriate protocol set, you can go to the Setup menu from any protocol. Note You cannot use the Xtain menus when the Processor Plus is running a protocol.

The Hoefer Processor Plus allocates memory for ten blot processing protocols, five of these are pre-programmed Table See page 17 for directions on editing protocols. The Processor Plus has two trays for blot processing Table One lid fits either tray.

Jun 02,  · But the good news doesn’t stop there—arguably, the best reason to choose gel stain is its ease of use. When it comes to application, gel stain requires less . Apply a thin, even coat of Gel Stain to a section using a natural bristle brush or a soft, lint-free cloth. Apply in the direction of the grain you intend to create. Graining: While the Gel Stain is wet, use a graining tool to create a wood grain pattern. Apply downward pressure while moving the graining tool across the section at a constant speed. may promote dye precipitation if the gel stain is reused. 3. Place the gel in a suitable container such as a polypropylene staining tray. Add a sufficient amount of the 3X staining solution to submerge the gel. 4. Agitate the gel gently at room temperature for ~30 minutes. Note: Optimal staining time may vary somewhat depending on the thickness.

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